JCM 5046 PDF

Bazahn Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing; 25th informational supplement. Open in a separate window. Comparison of phenotypic tests for detecting BKCproducing Enterobacteriaceae isolates. It is important to note that such new studies must search for bla BKC-1 using molecular techniques, since phenotypic tests may not accurately detect BKCproducing isolates 8.

Author:Kijinn Dilmaran
Country:Timor Leste
Language:English (Spanish)
Genre:Career
Published (Last):12 March 2006
Pages:310
PDF File Size:16.51 Mb
ePub File Size:13.48 Mb
ISBN:465-6-13219-610-5
Downloads:96134
Price:Free* [*Free Regsitration Required]
Uploader:Dasho



Bazahn Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing; 25th informational supplement. Open in a separate window. Comparison of phenotypic tests for detecting BKCproducing Enterobacteriaceae isolates. It is important to note that such new studies must search for bla BKC-1 using molecular techniques, since phenotypic tests may not accurately detect BKCproducing isolates 8.

Santosb Jorge L. The carbapenemase was encoded by bla BKC-1which was located on a small nonconjugative plasmid 2. These carbapenem resistance genes are frequently harbored on mobile genetic elements that are highly transmissible, contributing to the increasing frequency of carbapenem-resistant Enterobacteriaceae 1.

BKC-1 is a new class A serine carbapenemase that was recently identified in Klebsiella pneumoniae clinical isolates. Only 2 BKCproducing K. Martinsa Adriana G. Author information Article notes Copyright and License information Disclaimer.

In addition, bla BKC-1 was harbored by a small unstable plasmid that could be easily lost in the absence of selective pressure. Among the Klebsiella strains, We verified that, in the absence of selective pressure, the plasmid harboring the bla BKC-1 gene, p, was unstable.

Support Center Support Center. These data might suggest that, although genetically related, the isolates were probably subjected to distinct selective pressures, which could have resulted in different OMP modifications. Plasmid extraction of K. More studies with greater numbers of isolates from all Brazilian states are necessary to assess the real prevalence of isolates carrying bla BKC Methods for dilution antimicrobial susceptibility tests for bacteria that grow approved standard— 10th ed CLSI document MA To determine whether this plasmid was identical to p, the first plasmid identified as carrying bla BKC-1we performed whole-plasmid sequencing of the plasmid recovered from KpnJ.

Sampaiob, c and Ana C. The Klebsiella strains were isolated from different clinical specimens obtained between and Fig. Address correspondence to Willames M. To evaluate the stability of p harbored by the Kpn isolate, we performed serial passages of the Kpn isolate on Mueller-Hinton agar plates without the addition of antibiotic. BKC-1 Brazilian Klebsiella carbapenemase is a class A serine carbapenemase that was recently identified in Klebsiella pneumoniae isolates from Brazil that belonged to sequence type ST clonal complex [CC] 2.

Abstract BKC-1 is a new class A serine carbapenemase that was recently identified in Klebsiella pneumoniae clinical isolates.

Related Articles.

CSI 9420 PDF

JCM 5046 PDF

Nelmaran A total of Klebsiella strains collected from previous surveillance studies were randomly selected for this study, in order to represent distinct Brazilian geographic regions. Journal List Antimicrob Jcn Chemother v. Antimicrobial susceptibility testing was performed by the broth microdilution method, according to CLSI guidelines To determine whether this plasmid was identical to p, the first plasmid identified as carrying bla BKC-1we performed whole-plasmid sequencing of the plasmid recovered from KpnJ. In conclusion, prudent use of antimicrobials and strict adherence to infection control measures seem to be the only hcm tools for preventing the acquisition and spread of new carbapenemase-encoding genes. Only 2 BKCproducing K.

C51GM-M MANUAL PDF

Initially, we performed transformation by electroporation into Escherichia coli Top Since BKC-1 is a weak carbapenemase other mechanisms could be associated with carbapenemase production, we selected Klebsiella strains independent of the resistance phenotype. Published online Jul It is important to note that such new studies must jc for bla BKC-1 using molecular techniques, since phenotypic tests may not accurately detect BKCproducing isolates 8. Please review our privacy policy. Only 2 BKCproducing K. J Antimicrob Jfm sequence typing of Klebsiella pneumoniae nosocomial isolates. The Klebsiella strains were isolated from different clinical specimens obtained between and Fig.

DINE BIZAAD PDF

This article has been cited by other articles in PMC. However, Southern blot analysis revealed that the bla BKC-1 gene was located on the same kb plasmid in both isolates. Frequency of BKCproducing Ucm species isolates. These data might suggest that, although genetically related, the isolates were probably subjected to distinct selective pressures, which could have resulted in different OMP modifications.

Related Articles